Does anyone have primers for m2rtTA for mice genotyping?

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What analytical equations are used to calculate nuclear gradients in dft?

I'm looking for an explanation of how the calculation of nuclear gradients works in DFT in the context of geometric optimization. Mostly, I want to know what equations are used to calculate...

03 November 2023 8,144 1 View

How to remove dirt from diamonds for AFM study?

Hello everybody. I'm trying to study HPHT synthetic diamond plates with low (first nm roughness). When i taking 100×100 μm photo by AFM, you can see a lot of flakes/dirt on them. The question is...

25 October 2023 7,952 4 View

How to freeze atomic positions and set atomic charges when optimizing geometry in pybel?

I'm trying to do something like docking, but for crystals, in the task of transcribing XANES spectra, estimating the random positions of inserted atoms into a fixed crystal structure using a graph...

23 October 2023 3,850 0 View

What type of filter should be used when printing with titanium powder in an SLM printer?

Dear Colleagues! I would like to know your advice about printing with titanium powder in an SLM printer. Will a HEPA filter be enough to filter the gas from residual printing products when working...

20 August 2023 9,837 1 View

How can I automatically get charges of each carbon and nitrogen atom in skeleton of molecule after gaussian calculation?

Hi, I have a list of output files after a Gaussian calculation, and I want to get the charges of carbon and nitrogen atoms in the constant skeleton of these molecules to use them later as QSAR...

17 July 2023 6,438 1 View

What is the place of PLTs in the actual practice of foundation design in the US? What US documents regulate calculation methods?

Hello! I am doing a review on Plate load test in global practice. I have started by looking at foundation design practices in general and I have questions when looking at the US code system and...

25 June 2023 3,166 2 View

Litterature on China-ASEAN sustainable cooperation?

Dear all, cuurntly im a writing a graduation paper on the topic of cooperation between China and ASEAN in terms of sustainable development. I've already found quite a few official reports and...

02 March 2023 7,435 0 View

Tell me what the arrows in the algorithm mean ?

I would like to know the translation, what does the word mean "zoom" (translation needed word. I will be very grateful for the answer!

19 February 2023 1,569 0 View

Google Scholar Preprint Bug - any way to avoid?

The bug when published paper did not appear in Google Scholar after posting preprints at BioRxiv and ArXiv has been described in 2014:...

29 November 2022 6,857 0 View

What post-hoc is appropiate for repeated measures ANOVA?

Dear colleagues! I have two groups of animals which I compare using 8 day long behavioral paradigm. For each day I have two data points (day and night). Thus I have one independent factor (animal...

04 August 2021 4,490 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View