1H NMR of alkoxides in CD2Cl2?

Hello! I am planning to get an alkoxide which should be potentially soluble in CH2Cl2. Do you think it is okay to use CD2Cl2 to get an NMR spectrum of the alkoxide? I am wondering if RO- anion...

25 November 2020 1,819 1 View

Arrays in VBA errors?

Colleagues, I wrote a function in VBA to calculate the values of a two-dimensional array. The principle of operation (I want to) is as follows: 1. The values entered were read and written in a...

09 June 2020 7,690 3 View

How can I use Kramers-Kronig rel. to reflectance spectrum to receive the absorbance spectrum?

Hello everyone! I'm in trouble now. I've faced a problem that I can't solve. I need to receive the absorbance spectrum from the reflectance spectrum using KK in matlab, and I'm having some...

19 February 2020 6,154 3 View

Any literature on Pakistani military ideology?

Dear colleagues! Could you name some useful articles and / or books on the ideological and political development of the Pakistani armed forces, especially on the role of India in the process ? How...

25 January 2020 3,423 3 View

Help with VBA code for Gauss elimination?

Colleagues, I wrote VBA code for function of Gauss elimination (in attached files). But I can't launch this function in Excel. I can't find it by her name in line for function's input. Could...

19 January 2020 5,155 5 View

Is there any authomatic devise or method to measure a resistance from temperature dependence in semiconductors (VO2)?

For now, the method we use to measure resistance from temperature dependence in vanadium dioxide (VO2) is really slow: you need to look at oscilloscope and thermometer and write down the numbers...

28 June 2019 4,107 3 View

Any books on the cartography of the British Empire?

Dear colleagues! Would you kindly name books or maybe articles on the history of the cartography of the British Empire and / or spatial perceptions in it? How the British created maps of their...

06 March 2018 7,662 3 View

Are there any theoretical or experimental data about the low-temperature specific heat of paramagnetic solids with dipolar (not exchange) interaction?

The Schottky anomaly is well-studied, but it exists in ideal systems without interactions. What is the dependence of specific heat on temperature, if the spin-spin interaction is significant? I've...

18 March 2016 6,766 2 View

Protocol for micro chromatin Immunoprecipitation?

29 November 2014 6,051 2 View

What is the correct boundary condition at an impermeable wall for porous media flow?

The most common condition I have seen is vanishing of normal component of fluid velocity at the wall. It makes sense, because Darcy's law describes pore-averaged flow, and the fluid moves even...

30 November 2013 823 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View