Does anyone have or know about a protocol for isolating microvessels from mouse brain to study tight junction proteins?

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How can I use an hybridization of ontology and machine learning for text summarization ?

my work consists of creating a system for text summarization in a specific domain(crisis management) using a hybridization of ontology and machine learning algorithme ,my question is how to use...

23 February 2020 8,327 2 View

Gauge Pressure and Operating pressure on combustion system?

Hi all, Please I have a question about the pressure: I am working on combustion, and I want to study the effect of pressure in my geometry, I couldn't understand where sould I put the pressure for...

14 February 2020 7,977 3 View

What will happen if I add the NO models (NO thermal and NO prompt) for the flamelet model using GRI mech? How Fluent works in this case?

Hi everyone, The GRI mechanism contain all the reactions, and species of NOx, and in the Fluent we can also find the NO models ( prompt, thermal and N2O..), when I added the models I found a big...

22 December 2019 3,785 5 View

Is there a difference in binding site when comparing anti-CD279 (PD-1) clones EH12.2H7 and EH12.1?

Hi all, I would like to know if these clones of anti-PD-1 antibodies (EH12.2H7 and EH12.1) share the exact binding site. Thank you in advance for your help, Sabrina

19 March 2019 4,908 3 View

Is the title and goal of my project suitable?

I am starting works on a project with current working title: Gender Roles in African Philosophy: Appraising the place of women in African traditional society. A Case Study on Setswana and...

20 January 2019 2,161 4 View

How to fix the Error: The mesh file exporter could not resolve cyclic dependencies in overlapping contact regions?

How to fix the Error: The mesh file exporter could not resolve cyclic dependencies in overlapping contact regions. Please try to Repair Overlapping Contact Regions.

01 December 2018 7,320 3 View

What to use for correlation between ordinal scales?

Hello, I have a variable "firm's results" which is an ordinal scale and 5 variables for direct marketing, each of them in ordinal scale too. I know that I can use Pearson if I want to know the...

05 November 2018 1,535 3 View

I have tilt in my geometry, so Is there any way for doing Line moving with the flow ?

Dear professors and research scholars, I have a query for the code Fluent My geometry is not symmetric, I have tilt in my geometry so Is there any way for doing Line moving with the flow !!!...

19 February 2018 8,538 4 View

I have a problem with my results from FLUENT when I imported in ORIGIN, I have other lines! Does that mean the problem with the mesh or secondary air?

Dear professors and research scholars, Attached is a figure showing the distribution of the temperature along the central axis of combustor Fuel, I have problems with my results, When I put it in...

09 February 2018 738 2 View

Is it possible to perform two rounds of macs beads isolation?

I would like to isolate a subpopulation of T cells using magnetic beads. Is it possible to perform two positive selections in a row using macs beads and still get functional cells for culture?

18 November 2017 6,081 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View