Does anyone have MIN6 cells and Beta TC6-F7 cells that I can borrow for some experiments.

More Preetha Purushothaman's questions See All

Aurantiochytrium is growing faster while other strains are not. Why?

In case of Thraustochytrids, I got more than 6 isolates by agar plating. However except Aurantiochytrium spp. all others grow very slowly. What could be the reason behind? I am using GYP broth...

05 April 2024 7,486 2 View

Workflow to calculate screened/uncreened plasma frequnecy using DFT-VASP?

I've been working with DFT-VASP and have a few questions regarding the calculation of screened/unscreened plasma frequency. I'd like to outline my workflow and the parameters I've used, and I'd...

03 November 2023 9,915 1 View

How to calculate transport properties(carrier concentration and resistivity) for a metallic system using boltztrap2 and vasp?

Steps to Calculate Transport Properties for Metallic Systems with BoltzTraP2 and VASP I'm currently working on calculating transport properties, specifically carrier concentration and...

31 October 2023 4,535 0 View

Can I Please get the data set on Smartphone App Usage Analysis dataset?

20 October 2023 1,169 3 View

Is Design Engineering Scopus or fake journal?

I got this http://thedesignenginnering.com link through some friend. I didn't know that it was fake. I had submitted my paper. They have published it. But they aren't sharing me the full copy PDF....

30 October 2021 1,768 7 View

From which online source can I get a dataset for performing medical data encryption for testing an algorithm developed?

The aim of my work is to provide secure data sharing in a healthcare environment. I have tried a combination of Cipher-text Policy Attribute Based Encryption and the Blockchain technology to...

28 August 2021 3,740 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 9,077 3 View

Could you please advise me that any suitable techniques for this experiment which will be done shortly?

I am planning to perform the functional study of gene whuch is supplied maternally, so morpholino-mediated knockdown assay won't work. Also about the CRISPR mediated knockout study need to wait...

27 December 2020 889 1 View

While doing gradient PCR for my gene of interest, Iam getting multiple bands in including a amplified band lesser than the size of my interested gene?

I have checked my primer sequences and they are particular with my gene. What could be the reasons for this?

25 September 2020 3,582 0 View

How to go about designing primers?

I am working on gene sequencing for an organism that has little to none sequencing done. I have acquired the homologous sequence for that particular gene across three species. I would like to know...

24 August 2020 6,562 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View