21 October 2023 3 1K Report

Hi

More Preetha Attuparampath's questions See All
What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

While doing gradient PCR for my gene of interest, Iam getting multiple bands in including a amplified band lesser than the size of my interested gene?

I have checked my primer sequences and they are particular with my gene. What could be the reasons for this?

25 September 2020 3,398 0 View

How to go about designing primers?

I am working on gene sequencing for an organism that has little to none sequencing done. I have acquired the homologous sequence for that particular gene across three species. I would like to know...

24 August 2020 6,379 3 View

I have RNA sequencing data which i would like to analyse for expressions, which software should i use to study the gene expression?

this is my first time doing bioinformatics, so a user friendly software would really be good. Thank you.

08 June 2020 6,737 15 View

Are there any technical hints to have low cv% and high accuracy throughout the standard curve?

I have been having problems with my ELISA on standard curve. In particular, the lowest point on the standard curve seems to have a cv greater than 20%. The rest of the curve seems to be fine. I...

25 January 2015 5,535 5 View

research phytochemicals

I am interested in working with phytochemicals, mainly derived from moringa oleifera or jambulana jambulana.

01 July 2010 1,163 3 View

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03 March 2021 4,116 4 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone have kinetic and kinematic dataset for gait classification?

I want to differentiate between normal and abnormal movement. For this reason, I am looking forward to this type of dataset.

01 March 2021 9,485 1 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

How to download dataset?

I have to download dataset from software infrastructure repository (SIR) and I have created account on repository but I am not able to download plz guide.

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