I have checked my primer sequences and they are particular with my gene. What could be the reasons for this?
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 8,618 3 View
Please help me to find the latest works.
30 September 2020 977 8 View
I am working on gene sequencing for an organism that has little to none sequencing done. I have acquired the homologous sequence for that particular gene across three species. I would like to know...
24 August 2020 6,379 3 View
this is my first time doing bioinformatics, so a user friendly software would really be good. Thank you.
08 June 2020 6,737 15 View
I have been having problems with my ELISA on standard curve. In particular, the lowest point on the standard curve seems to have a cv greater than 20%. The rest of the curve seems to be fine. I...
25 January 2015 5,535 5 View
Weak Volterra space
19 January 2014 8,787 2 View
Titanium maching
16 December 2011 8,700 2 View
I am interested in working with phytochemicals, mainly derived from moringa oleifera or jambulana jambulana.
01 July 2010 1,163 3 View
Looking for Master thesis inspiration reflecting the above mentioned title - I wish to conduct a empirical study in collaboration with an international bank's IT department. However looking for...
01 January 1970 5,811 3 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?
28 February 2021 1,254 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View