I have checked my primer sequences and they are particular with my gene. What could be the reasons for this?
In case of Thraustochytrids, I got more than 6 isolates by agar plating. However except Aurantiochytrium spp. all others grow very slowly. What could be the reason behind? I am using GYP broth...
05 April 2024 7,486 2 View
Hi
20 October 2023 1,169 3 View
There were common themes across question and instead of switching response to appropriate question, answers were coded across questions. Is it appropriate to use inductive approach in this case?
05 December 2022 8,225 1 View
I got this http://thedesignenginnering.com link through some friend. I didn't know that it was fake. I had submitted my paper. They have published it. But they aren't sharing me the full copy PDF....
30 October 2021 1,768 7 View
The aim of my work is to provide secure data sharing in a healthcare environment. I have tried a combination of Cipher-text Policy Attribute Based Encryption and the Blockchain technology to...
28 August 2021 3,740 3 View
Hello, I really have no experience with how to prepare PVA/GA films. These films will need to be spincoated to be ~200-500nm thick. I have PVA (avg weight 85,000-124000, 87-89% hydrolyzed) and...
08 June 2021 1,594 2 View
which is the best method for analysis of lipopeptides secreted by the bacteria (bacillus sps.) whether HPLC, LCMS, LCMS/MS, LC-ESI-MS or GCMS?
07 March 2021 6,364 6 View
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 9,077 3 View
Please help me to find the latest works.
30 September 2020 1,120 8 View
I am working on gene sequencing for an organism that has little to none sequencing done. I have acquired the homologous sequence for that particular gene across three species. I would like to know...
24 August 2020 6,562 3 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View