Does anyone have manual for Bio-logic RK 300 patch-clamp amplifier?

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Can you adjust MiniAmp Thermal cycler cover gradient?

I was wondering if anyone had issues with their MiniAmp. Overall, I'm quite happy with the instrument but I find the lid heats/cools extremely slowly (0.3 degrees/sec) and I cannot find any way to...

07 July 2022 7,476 0 View

RT-PCR systems for SARS-CoV-2 identification: SLAN 48p vs. AGS 4800?

Hi, Everybody, I'm looking for someone with expertise with Zeesan's Lab-Aid 824s Extraction System and/or SLAN®- 48P RtPCR (especially with the SARS-CoV-2 kit). What are your in-practice...

08 May 2020 8,246 0 View

How to obtain similar specific growth rate value of linearisation with modified Gompertz model fitting?

I am not satisfied with the method of linearisation of microbial growth curve to calculate the maximum specific growth rate (ln(x1-x0)/(t1-t0), because to find the linear part of the ln curve is...

06 December 2019 6,149 4 View

What is the ratio of 3D/3+2D/5D milling technology?

I am looking for a survey or report about the application ratio of 3D/3+2D/5D milling technology in the industry.

18 December 2018 7,021 2 View

Modelling predator-prey interactions

We intend to explore predation rates on snakes by using clay models but "ordinary" plasticine melts when exposed to heat. Can anyone recommend a suitable brand of modelling clay that does not lose...

14 August 2017 1,878 4 View

Would you have any idea how to transform the wavefunction as such into an informational onservable in quantum algebraic sense?

I am contemplating about such a formalism as regards to introduce the wave side of the dualism in a much more explicite way than is done in orthodox QM, in which the "informationally"...

03 February 2017 2,280 22 View

In what deeper sense can you speak about the quasiclassicality of the DNA macromolecule?

You can see under an ordinary light microscope that a DNA polymer is nearly a macroscopic object. But in a strict quantal sense can we expect quasiclassical behaviour, i.e., describability by a...

03 February 2017 7,868 2 View

Which types of natural clays and zeolites are the most effective adsorbents for phenol and PAH?

Many researchers agree that chemically modified clays and synthetic zeolites can be useful treatment for wastewaters polluted by phenol and/or PAH compounds. But which are the best tested natural...

22 May 2016 9,098 1 View

Which value of RNA Integrity Number (RIN) is considered suitable/sufficient for qPCR?

Which value of RNA Integrity Number (RIN) is considered suitable/sufficient for qPCR? Thank you

21 April 2016 2,111 3 View

Any advice in choosing MoBio PowerSoil vs. PowerMax Soil DNA Isolation Kit for fungal pyrosequencing?

We have sandy soil samples with quite patchy fungal communities. At least I got a bit different ITS T-RFLP profile for three parallel PowerSoil DNA samples. And when I made a fourth isolation with...

12 December 2015 3,854 6 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

05 August 2024 5,829 1 View

Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

How can we identify (in silico) the interacting amino acid residues or the nucleotides involved in the Protein-Protein / Protein-RNA interaction?

Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...

14 July 2024 950 2 View