Does anyone have knowledge of the efficacity of TaKaRa Ex Taq Hot-Start Version kit in large spectre PCR for diagnosis?

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Is it possible to get good sequencing results despite having no signal on electrophoresis gel ?

I was performing a 16Srrna Broad Range PCR. Despite having no signal on my electrophoresis gel, I still tried to elonguate and sequence ( Conventional Sanger sequencing ) to get results. I was...

10 November 2018 7,928 6 View

Any advice on the standard for Colony PCR on direct samples ?

Hi Folks, We performed colony PCR on 16S eubacterial gene for a long time, since a bit of time we got small issues with our positive controls, the band aren't so bright and beautiful as before. We...

11 December 2015 6,630 9 View

Does any one has knowledge why phagotherapy is so uncommon in western Europa countries?

Since I read about this on some articles, I can't stop asking myself why such techniques are not widely used in Western Europa Countries? It seems that it is very efficient in Eastern countries...

08 September 2015 7,944 3 View

How can I increase the performance of my Eubacterias PCR on Patients sample?

I explain, i'm doing PCR on Chlamydia trachomatis. The PCR on strains works fine, i got large bands on gel, and when i'm sequencing it, the result is perfect.Then i had to do the PCR on patients...

01 February 2015 9,149 5 View

What type of Mix (Roche, Applied biosystems, ...) could I use to optimize the time of my home made PCR ( Eubacteria, Mycobacteria) ?

I'm doing work on a new home made PCR for diagnostic use (large spectre detections for eubacteria, mycobacteria, identifications & resistances genes) I need to optimize the time and cycle to...

09 October 2014 6,184 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View