B.A. Padder

6 Questions 68 Answers 0 Followers

Questions related from B.A. Padder

Need of Agrobacterium AGL-1 strain with pBHt2 vector for Colletotrichum lindemuthianum transformation?

I want to start fungal transformation for identification of pathogenicity genes in colltotrichum. for this I am in need of AGL-1 strain with pBHt2 vector. Is any one having it and could spare it...

01 January 2016 9,238 3 View

Can anyone tell me the steps of JoinMap 3.0 for making a genetic map along with the precautions I should take?

I am having SNP data of RIL populations and the Phenotypic data. Now I want to construct the linkage map using JoinMap. As I have no expertise of join map it would be nice if someone could provide...

12 December 2014 5,117 8 View

How long is it possible to store cDNA at -40c?

For how much time cDNA can be stored at -40 deg. Is it possible to store cDNA for nearly 6 months or above? Thanks for advise in advance

07 July 2014 7,294 21 View

Anyone have suggestions for separating SSR on Metaphor Agarose?

I am trying to separate the SSR markers of Venturia Inaequalis (Apple Scab Fungus) having Bp range of 171 t0 180 (maximum difference of 1bp to 7 bp). But, while separating using Metaphor Agarose,...

01 January 2014 4,710 9 View

Can anyone suggest how to do standardization of EST SSR's?

I designed SSR markers using MISA tool from the EST database downloaded from NCBI. The product size of one of the markers which I amplified in the PCR shows no specific binding. I also did...

11 November 2013 4,736 1 View

Primerpro tool?

We are trying to do Esr SSR analysis from a FASTA file using primer pro, it works nicely for "example file included in documentation " but when applied to our own multifasta file it shows some...

09 September 2013 8,994 1 View