Atefeh Mohammadi

5 Questions 6 Answers 0 Followers

Questions related from Atefeh Mohammadi

Can you do PCRs to detect inflammatory cytokines/chemokines in conditioned media?

I want to expose my lung epithelial cells to different treatments in vitro and then detect pro- and anti-inflammatory cytokines/chemokines in the conditioned media - can I then do PCRs on the...

19 May 2022 2,638 4 View

Can you use 4% formaldehyde instead of paraformaldehyde when fixing cells for IF/ICC?

I would love to get some insight as to whether it is possible to use 4% formaldehyde instead of paraformaldehyde when fixing cells for immunocytochemistry/ immunofluorescent staining? Thanks!

21 April 2021 5,719 5 View

How do you passage cells seeded on coated plates?

What is the best way to passage cells seeded on collagen I/ fibronectin plates? Is it sufficient to just use trypsin-EDTA/ an equivalent (eg. Tryple Express/Accutase)? Can you use a cell scraper...

15 April 2021 2,252 2 View

Is it possible to passage cells seeded in transwell plates? If so, how?

I am wondering if cells seeded in transwell plates can be passaged? If so, how (just trypsin/equivalent?)? If not, are cells supposed to be seeded in non-transwell plates first and then seeded in...

15 April 2021 1,605 1 View

Does anyone have insight on the isolation and cell culture of Type I Alveolar Epithelial Cells?

I am looking for isolation (FACS) and cell culture protocols for rat Type I alveolar epithelial cells. I have read "Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat...

10 January 2021 834 0 View