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Questions related from Atefeh Mohammadi
I am looking to measure lung wet/dry weight ratio and do the Evan's blue permeability assay in neonatal rats treated with LPS (inflammatory injury) and drug treatment to determine the...
05 December 2024 887 0 View
I want to expose my lung epithelial cells to different treatments in vitro and then detect pro- and anti-inflammatory cytokines/chemokines in the conditioned media - can I then do PCRs on the...
19 May 2022 2,741 4 View
I would love to get some insight as to whether it is possible to use 4% formaldehyde instead of paraformaldehyde when fixing cells for immunocytochemistry/ immunofluorescent staining? Thanks!
21 April 2021 5,758 5 View
What is the best way to passage cells seeded on collagen I/ fibronectin plates? Is it sufficient to just use trypsin-EDTA/ an equivalent (eg. Tryple Express/Accutase)? Can you use a cell scraper...
15 April 2021 2,299 2 View
I am wondering if cells seeded in transwell plates can be passaged? If so, how (just trypsin/equivalent?)? If not, are cells supposed to be seeded in non-transwell plates first and then seeded in...
15 April 2021 1,670 1 View
I want to use RBC lysing buffer to remove RBCs from my primary rat lung cell suspension prior to cell sorting (FACS). I am using the BD Pharm Lyse buffer- I'd appreciate any tips or advice!
05 April 2021 1,301 4 View
I am looking for isolation (FACS) and cell culture protocols for rat Type I alveolar epithelial cells. I have read "Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat...
10 January 2021 888 0 View
