Does anyone have experiences with culturing Pre B cell (15-79-2 (ATCC® HB-34™))?

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What are the frameworks or methodologies to examine written academic ELF?

What should be the frameworks or methodologies to examine written academic ELF? I want to explore the linguistic features of written ELF in research articles.

23 July 2024 4,800 1 View

Does it work as antibiotic resistance if plamid has no RBS??

I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...

11 July 2024 7,847 1 View

How to analyse response rates of a questionnaire?

I have developed a questionnaire. I want to try and analyse question drop-off. There is a clear negative correlation between question number and percentage of respondents who answered that...

07 July 2024 9,435 1 View

Can anybody recognize the virus on potato plants?

Infected potato plant was negative for PVY, PVX, PVM, PVS. What can it be?

19 June 2024 6,739 3 View

Is the preference for more established researchers by reputable academic journals leading to infrequent publishing of Bachelor's degree theses?

I think, for a long time, there has been a tendency for journals to favour work from more established researchers or institutions, thus potentially overlooking quality undergraduate research. This...

16 June 2024 6,801 3 View

How do you generate recursive proofs over time series data?

What are the recommended recursive algorithms to generate proofs of time variant and time invariant properties from a time series data. Approaches like recursive statistical tests, symbolic time...

08 June 2024 4,449 0 View

What is the ratio between the density of the bacterial population during anaerobic and aerobic growth of one species?

What is the relationship between the maximal density of a bacterial population during anaerobic and aerobic growth for a specific species - a facultative anaerobe?

01 June 2024 4,950 4 View

Why do Spanish slugs (Arion vulgaris) prefer to feed on the most poisonous or spicy plants?

Spanish slugs choose to feed on some of the most poisonous and spicy plants. Do they become toxic to potential predators due to this? Are there any publications available on this topic?

28 May 2024 3,929 0 View

Any low-cost mid-infrared spectrometer?

If we build up a mid-infrared laser system and want to measure its spectra, where can I find the lowest-cost mid-infrared spectrometer, ranging from 2 to 20 μm。 Any recommendation? Thanks!

26 May 2024 8,114 1 View

What is the meaning of intersection point in transfer curve ?

Hi, I'm doing research n-type neuromorphic transistor device. According to theory and other research, forming EDL (electro double layer) can cause anti-clockwise transfer curve. However, as you...

26 May 2024 5,402 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View