What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Does anyone know why I would have unexplained emission overlap for flow cytometry?

Hello, I am new to flow cytometry, and was just using a sample cell population to train with the technician. The cells were PAC-1 cells split into four groups. Unstained, transiently transfected...

28 January 2021 969 2 View

Does anyone know of a library for R that includes phi?

I'm looking to produce some phi correlations and I'd rather not do it manually, but I can't find a library for R that includes phi.

18 January 2021 2,808 9 View

In SEM, how to interpret standardised regression weights and their significance?

I have a SEM model that I'm having difficulty interpreting. It has one direct effect with a Beta/standardised regression weight that appears to be high (0.80) and significant (p

31 December 2020 7,604 6 View

Does anyone know how to add custom text to a violin plot in r, ggpubr made using the ggviolin line?

I've produced a violin plot for a point-biserial correlation. Main title is there, x and y are labelled, but I want to add a custom text box with the correlation coefficient and significance...

26 December 2020 4,480 4 View

How do I interpret these correlations and p-values?

I've just run a series of point biserial correlation tests in R between whether or not characters were assigned national identities, and attributions given to their behaviours - results shown in...

17 December 2020 9,825 4 View

Is it possible to fuse Deep Learning Net and Clustering Algotrithm?

What I did (unpublished yet) is extract several (e.g. 16) mask features within Mask R-CNN from multiple images, construct a distance metric to compare them to predict the image class. Each set of...

13 December 2020 9,504 2 View

Where can I find fetal MRI dataset?

I want to find the fetal brain MRI dataset for cnn model and deep learning.Can anyone help me, thank you very much

09 December 2020 9,966 2 View

Are there models trained+evaluated on multiple datasets?

Generalization of COVID-19 models is a very serious challenge. I'm trying to find a model (ct scans or x-rays) that was trained and evaluated on multiple datasets. It would be particular...

03 December 2020 8,863 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View