How to do a repeated measures ANOVA with two within factors, where one factor is random and the other fixed?

We performed an experiment on a number of blood donors. We took the blood from each donor and divided it into 9 equal volumes. Three of the blood samples from each individual were left as...

17 May 2020 9,326 5 View

Hypothesis test for conditional mean?

I have a single time series which I have computed a mean for. I am interested in comparing this time mean against one computed from the same time series, but with a few of the most extreme values...

13 September 2019 9,736 9 View

Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies?

Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies? We have tried several available antibodies for use in human biopsies, but none are...

24 February 2019 6,461 3 View

Survey containing audio?

I'm looking for survey software that contains audio (mp3) files for quantitive purposes, does anyone know of such software?

02 March 2018 7,212 3 View

Excessive boiling of proteins for Western Blot?

HI all. I was preparing my samples for Western blot, and accidentally boiled my proteins at 100C for around 8 min instead of the intended 5 min. My proteins of interest did not show up on the...

01 February 2018 4,504 3 View

Can anyone recommend good antibodies for NF200, CGRP, or isolectin B4?

Will be completing immunofluorescence experiments in formalin fixed mouse spinal cord/DRG and wanted to know if anyone could recommend antibodies for NF200, CGRP or IB4 that they've used in the...

31 January 2018 9,500 4 View

Does the expansion of the universe happen at the same rate at all points in space?

I posted a similar question earlier but framed it poorly. I understand that the redshift increases with distance. My question is whether the local expansion rate, the expansion rate at a point, is...

07 March 2016 1,902 32 View

Is the universe expansion rate constant in space at any given time, including at quantum scales?

My understanding is that the cosmological constant, though poorly understood, is assumed to be uniform in space. Does this uniformity extend to small scales or is it only uniform at very large...

28 December 2015 5,430 35 View

Any advice on pressure distribution in fluent?

I need to determine the pressure distribution around a cylinder that is being subjected to external flow. How can I do this?

22 October 2015 2,278 1 View

How can we minimise / avoid autofluorescence of Eosinophils in Hodgkin tissue?

We are performing multiplexed immunofluorescence on formalin-fixed, paraffin embedded Hodgkin lymphoma. A major issue has been autofluorescence or non-specific binding of what we think are...

19 November 2013 548 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View