Martin Helley

3 Questions 5 Answers 0 Followers

Questions related from Martin Helley

What is a reliable protocol for processing cell monolayers for transmission electron microscopy?

I have been trying to process neuronal cells grown as a monolayer on glass coverslips for standard TEM but my images are not to the standard I was hoping for (see attached). It looks to me like...

28 October 2015 3,703 9 View

How do I present my qPCR data?

I am using real-time PCR to firstly show the presence/absence of certain targets in my tissue and also to then give more information of the relative amounts of these targets compared to each...

16 June 2014 7,526 2 View

Why are my DNA bands smearing in the heavy direction?

I have loaded my qPCR products onto a 2% agarose gel and ran them at 60V for 1 hour. I ran 2.5ng of cDNA in a 10uL qPCR reaction. As you can see in my image there is smearing of all bands in the...

13 June 2014 4,917 2 View