Michael Scheid

3 Questions 19 Answers 0 Followers

Questions related from Michael Scheid

For a SNF2-subfamily type helicase, what is the most common approach for killing helicase activity?

What is commonly done to render the enzyme inactive, ie, a mutation in the ATP binding domain, etc...

02 February 2020 9,582 4 View

Immunofluorescence with three antibodies/colours?

I need some advice on the following experiment. We have fixed cells on coverslips that we need to examine with three specific antibodies. One is rabbit, two are mouse. I can stain the rabbit...

10 October 2018 4,430 3 View

What is the best internal control for normalizing qRT-PCR for fibroblasts stimulated with serum?

We amplified with oligo dT, so we can't use 18s rRNA. Has to be mRNA. What doesn't change with serum?

03 March 2014 9,582 7 View