Christopher Lee Fogarty

4 Questions 22 Answers 0 Followers

Questions related from Christopher Lee Fogarty

Anyone have any experience with Qiagen's AllPrep DNA/RNA/Protein kits?

I haven't used them before and was wondering if the RNA and DNA protein yields have been roughly equivalent to those obtained from single prep isolation (eg 5''s DNA extraction or Qiagen's RNease,...

01 January 2014 7,239 0 View

How can I obtain RNA from neutrophils after ex vivo stimulation in whole blood?

I am looking for a way to obtain RNA from neutrophils after a whole blood stimulation. Cell sorting via FACS is one option, but I wonder if anybody has tried percoll isolation immediately...

12 December 2013 7,244 2 View

Does anybody have experience in intracellular cytokine or NFkB staining for FACS in human neutrophils?

Neutrophil permiablization seem to be a bit more tricky than in other cell types.

09 September 2013 3,364 2 View

Inactivating autologous serum for PBMC experiments?

I am looking at activation states in certain PBMC subpopulations. My culture media includes autologous serum. I have been heat inactivating it, just in case, but couldn't really make a good...

08 August 2013 5,424 14 View