Hi everyone, I have been using magnetic nanobeads (Chemicell) as a biomaker probe to test C-reactive protein in human serum or plasma by MALDI TOF MS. When I test commercial serum sample which is also from human serum with high purity of C-reactive protein, I could get good result with nice spectra.
However, when the real human serum or plasma, fresh prepared in our lab, are tested in the same way, there are typical interference peaks at 7.7kDa and 8.2kDa. I have tried different kinds of blocking buffer and washing buffer to eliminate the impact of non specific binding, but totally failed. Does anyone have such experience in this field to give some advice? Or what kind of blocking and washing buffers are more efficient?
I do not have a clue to your problem,But i am almost experiencing the same issue but at the level of spectrophotometry
i am using magnetic nanoparticles as a traetment to my cells...
when i want to measure protein content of the cell lysates containing magnetic nanoparticles , they never give correct reading "Absorbance" at 595 nanometer wavelenghth.
it gives false high positive reading not correlating to actual protein concentration
till now i am unable to fix this issue!!
I previously worked on Magnetic nanoparticles, from that view I can say magnetic nanoparticles are flexible for drug targeting (Targeted drug delivery system) but some times its used in analytical purpose. For your study you should to use Ferrogel (Magnetic Gel). Dont use the solid Magnetic nanoparticles.
May be by this way you can try.
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