Acid blue 92 is not a standard dye for SDS- PAGE gel.

Article Standard Dyes for Total Protein Staining in Gel-Based Proteo...

You can use Visible dyes (Coomassie blue or silver staining).

https://www.chem.ufl.edu/wp-content/uploads/sites/17/2014/05/SDS-PAGE-Stain-Protocol.pdf

Bhairavi Vajaria, Thank you for your quick response.

You are absolutely right. Acid blue 92 is not a standard dye for SDS- PAGE, but according to the European Pharmacopoeia we must determine protein purity using SDS-PAGE with acid blue 92 staining.

Natalya Misiuk,

Going out on a limb here.... but when I looked up the European Pharmacopoeia on protein purity and SDS-PAGE, I found only a reference to "Coomassie", not specifically Acid Blue 92. A little more digging, and I learned that Acid Blue 92 was originally referred to as "Coomassie Blue RL" (CI 13390) but the term Coomassie has now been applied non-specifically to several different variations of blue dye. I'm guilty of using this generic reference.

I am likely incorrect, but I will ask nonetheless. Are you sure it has to be Acid Blue 92 and not Acid Blue 83 ("Coomassie Brilliant Blue", CI 42655) or one of the other acid blues (Coomassie G-250, Coomassie R-250)?

Lastly, I also found the abstract to this article which may also hold some answers.

Wittig, I., Braun, HP. & Schägger, H. Blue native PAGE. Nat Protoc 1, 418–428 (2006). https://doi.org/10.1038/nprot.2006.62

Unfortunately, I do not have full access to this journal. However, if you do, it may help point you in the right direction.

Kind Regards,

Melissa