Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Is it possible to full-couple the interFoam solver with the chtMultiRegionFoam solver?

We need to calculate multiphase stream of the one substance in the air atmosphere (this is why we using interFoam), but also we need to get heat transfer data between the substance, solid obstacle...

11 January 2021 872 2 View

Does anyone have experience with Land use/land cover data extraction from Second military survey maps?

I am trying to create polygon shapefile with land use from Second military survey using Arcmap. However, I really have hard time with reading the map, more correctly with boundaries between...

07 November 2020 6,018 3 View

How do I remove Bacillus sp. spores from Kraft Paper machine?

In the pulp and paper company that I work in, samples from paper, water and pulp from the paper machine gave information about existing spores of Presumptive Bacillus cereus. Our paper is used...

03 November 2020 1,726 10 View

What volume of a cell lysis buffer does one use for cells?

I have certain cells that are contaminant cells in a suspension of cells. I found a buffer that selectively removes them, but a lot of protocols don't specify what volume one should use for that...

03 November 2020 6,330 3 View

Best protocol to perform Western Blot from mice brain?

I am trying to look at the expression of c-fos nuclear protein in mice brain using WB technique. Can anyone suggest a protocol to extract the proteins? Better harsh homogenate before sonication?...

27 October 2020 5,272 2 View

Checking purity of sperm suspension?

I have sperm cells extracted from epididymis of a mouse. I would like to check and quantify their purity. My first idea is to use an intercalating dye like Hoechst and then see what fraction of...

25 October 2020 8,600 1 View

What do you think now of virtual teaching compared to face-to-face teaching of finished transmission of knowledge?

21 September 2020 4,178 10 View

How to store W/O droplets for over 10 days using paraffin oil with Span 80?

I am currently using a PDMS chip to produce water droplets with a diameter of 200 um in paraffin oil with 3wt%. After a couple of hours, the stationary droplets stored in the chip starts to form...

18 August 2020 2,459 3 View

How do you use factor analysis on categorical variables?

Hi, I'm trying to identify how similar different individuals are in their body traits. Normally, I would use a PCA or DFA, depending on my question. However, I have only categorical variables...

20 July 2020 4,171 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View