I am starting a new RNA-seq based project. I will be looking at both coding, and non coding RNAs. To enable me to look at both of these RNA species simultaneously, I will be ribo depleting the samples to remove rRNA. My question is will my RNA-seq data have enough coverage if I want to look at splicing changes. I realise when people usually do this sort of analysis they Poly A enrich samples for mRNAs. I could split the samples in two and do both ribo depletion and poly A enrichment independently but this would cost double for RNA seq. Any help or ideas much appreciated.