Ross A Cordiner

3 Questions 3 Answers 0 Followers

Questions related from Ross A Cordiner

Protocol for chromatin associated RNA isolation from frozen cells?

Does anyone know of a protocol which I can isolate chromatin associated RNAs from frozen cells. Essentially I am looking to monitor transcriptional output of RNA polymerases during a time-course...

23 October 2018 7,072 2 View

Does anyone have any experience with BrU-labelled RNA IPs?

I am currently metabolically labelling nascent RNA, however I am having problems eluting the BrU-labelled RNA from the beads. I am heating the beads to 95C for 10 mins. Also I am having trouble...

11 February 2016 7,485 1 View

Does anyone have experience with mRNA coverage with Ribo depletion in RNA-seq?

I am starting a new RNA-seq based project. I will be looking at both coding, and non coding RNAs. To enable me to look at both of these RNA species simultaneously, I will be ribo depleting the...

06 March 2014 3,470 1 View