I wanted to know if there is a way to quantify protein amount after IP. I want to run an invitro kinase assay using protein thats IP'd from cell lysate. I am expressing various mutants of the protein separately. How do you go about making sure you have approx. same protein amounts from each IP?
Hi Tamjeed,
for an in vitro kinase assay using proteins after IP I can recommend following publication: Wu, X et al., Molecular & Cellular Proteomics 12:2856-2873
Hi Tamjeed,
for an in vitro kinase assay using proteins after IP I can recommend following publication: Wu, X et al. (2013), Molecular & Cellular Proteomics 12:2856-2873
Hello amjeed,
Regulation of Chk2 ubiquitination and signaling through autophosphorylation of serine 379. The paper is for your reference.
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