Tamjeed Saleh

4 Questions 30 Answers 0 Followers

Questions related from Tamjeed Saleh

How to find the DNA/protein sequence of a yeast protein?

I am trying to find out what the DNA/protein sequence is for a mutant form of the yeast protein Sir4. The mutant form is called Sir4-9. Tracking down the original paper where the mutant was...

28 March 2018 1,467 2 View

Does anyone have experience with Kinase assays from immunoprecipitated proteins?

I wanted to know if there is a way to quantify protein amount after IP. I want to run an invitro kinase assay using protein thats IP'd from cell lysate. I am expressing various mutants of the...

30 September 2014 7,653 3 View

Is anyone familiar with the reaction conditions for in vitro myristoylation of a peptide/protein?

I am looking for buffer conditions; and type of NMT (recombinant).

15 October 2013 9,344 0 View

ADP glo (promega kit)?

I wanted to know if anyone has used a TECAN m200 for ATPase activity assay. I want to use it to measure ATPase activity of a kinase.

26 September 2013 7,345 3 View