Calculate pH after addition of base?

04 February 2021 2,232 4 View

Western blot with very acidic proteins, any suggestions?

I am trying to do a Western Blot to detect acidic proteins (~30kDa) that have a PI of ~3.5, due to the presence of Asp and Glu repeats. The proteins are about 80% pure. After I ran them on...

24 January 2021 7,226 3 View

How does one create IFORM contours?

Hello: Can anyone assist with plotting IFORM contours. I have been trying to plot contours using Hs and Tp as variables. The marginal distribution is a GPD-Kernel density and the conditional...

12 December 2020 4,355 3 View

How can I transfer heat between groups in Gromacs?

Is it possible to transfer heat from one group to another using simulated aneeling technique in the Gromacs software? I would like to transfer heat from a gold nanoparticle (group 1 from 310 to...

20 November 2020 9,062 3 View

Would ER NiCrMo-3 be the best option for weld overlay of carbon and low alloy steels?

Alloy 625 is the most popular alloy for weld overlay. Without considering the price factor, would NiCrMo-3 be the best technical option for weld overlay of API 5L and low alloy steels, in general,...

24 August 2020 2,478 4 View

Dose-response on non-Michaelian response enzymes, how?

Hello For typical dose-response assays, our lab usually uses steady state intervals for defining the difference between control and tested compound. For those assays, we typically use the angular...

23 July 2020 8,662 2 View

What is the best way to graph an SVM classification in R or Python?

I'm trying to find a way to graph a classification made by the support vector machine algorithm. Something like a PCA or LDA chart would be excellent. Any help will be welcome.

26 April 2020 4,588 1 View

Gene therapy with transgenics viruses Covid-19?

Viral load and symptomatic picture Is there any relationship between the viral load of exposure and contamination, in number of viruses and contamination processes, and the symptomatic picture in...

08 April 2020 6,349 2 View

Is it allowed to publish part of a journal paper(under review) as a conference paper?

Is it allowed to publish part of a journal paper under review as a conference paper? I have submitted a full research paper to a journal. I'm waiting for the review answer and I want to submit...

01 April 2020 430 6 View

Do KG-1a human macrophages need to be activated in some way?

Hello everyone. I was wondering if you can help me clarify this question. I am seeking to perform infection assays with bacteria, using this human cell line. Research articles regarding infection...

24 March 2020 9,884 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View