Dear Rundk

Please see this article:

Rapid Isolation of Type II Pneumocytes with Magnetic Removal of Macrophages

1983, Vol. 4, No. 3 , Pages 191-204

PDF (851 KB)

PDF Plus (385 KB)

Reprints

Permissions

W. Mark Lafranconi, Richard D. Spall, I. Glenn Sipes, Raymond C. Duhamel, Elias Meezan, and Klaus Brendel

1From the Department of Pharmacology, College of Medicine, University of Arizona, Tucson, Alabama

2Arizona and the Department of Pharmacoloy, University of Alabama in Birmingham, Birmingham, Alabama

A method is described for the rapid preparation of lung cell fractions enriched in type II alveolar pneumocytes. Isolated perfused rabbit lungs are exposed to Fe3O4 by tracheal lavage, which permits pulmonary alveolar macrophages to phagocytize the particles. Alveolar epithelial cells are then selectively freed from the basement membrane matrix by critical placement of collagenase and elastase. Detached cells are harvested either by repeated tracheal lavage or by mincing the lobes and filtering freed cells through a series of nylon mesh sieves. Iron oxide-containing macrophages are then removed from the harvested cells by a strong magnetic field. A final sizing of the macrophage-depleted suspension yields a preparation enriched in alveolar type II cells. Eight million viable cells (95% type II) were obtained per rabbit lung when harvested by lavage, while 32 ± 106 (88% type II) cells were obtained from minced lungs. These values for cell yield and relative purity are comparable to previously described separation methods that depend upon differences in cell density or size. A major advantage of the magnetic separation procedure is the substantially shortened preparation time, typically 2 hr instead of 4. The viability (90–95%), oxygen consumption (88 nmoll 106 cells/hr), and incorporation of [14C]acetate and [14C]choline (0.44 and 0.115 nmoll 106 cells/hr, respectively) indicate that these cells will be suitable for pharmacologic and toxicologic investigations.

Also see http://www.biomagres.com/content/4/1/4

Read More: http://informahealthcare.com/doi/abs/10.3109/01902148309046060?journalCode=elu

Hello

I hope you carefully read this study , which used the two types of virus strains ( BJx109 (H3N2) and PR8 (H1N1) ) . the results show that influenza viruses differ markedly in their abilities to infect murine macrophages in vitro and that infection of macrophages is nonproductive and no infectious virus is released.Depletion of airway macrophages by clodronate-loaded liposomes led to the development of severe viral pneumonia in BJx109-infected mice but did not modulate disease severity in PR8-infected mice.

ref ( Critical Role of Airway Macrophages in Modulating Disease Severity

during Influenza Virus Infection of Mice )

you can see all artical in attachment .

Good luck .

Dear Ravi!

Thanks for your explanation but i was woundering about invitro macrophage deletion by Clodronate liposome.

Dear Saleh!

Thanks so much that is very useful.

you are welcom 

Best wishes 

Thanks the same to you!