Does anyone have experience with DCFH assay in tissue (3D model, mice..)?
I'm making my research on metagenomics and I want to find the source of the command 'vsearch merge-pairs' that used to be 'vsearch join-pairs'' How can I cite that?
28 June 2024 3,960 1 View
Hey! I'm looking for ways to compare two kinetic curves over time. My data is composed of neutralizing antibody results during 11 points (vaccine kinetics) in each group (control and...
29 February 2024 9,979 3 View
Hello, We designed a series of tests that aim to assess knowledge in first-year university students, and went through the process of validating each one of them (construct validity, through EFA,...
01 January 2024 4,251 4 View
Hello everyone, I'm currently working with chromatograms on a Shimadzu system and am seeking assistance in converting files saved in either .CDF or .qgd formats to .d format for use in Mass...
12 December 2023 5,663 2 View
I am currently in the process of doing a Western Blot with mESCs after an AHA Assay. I added 25uL of 2xLaemmli buffer and 25uL of AHA buffer (50mM Tris HCL) to my pellet. I usually denature my...
05 December 2023 4,334 3 View
Hi everyone! I am new in lab and I have been having problems with Western Blot, I use a Chemidoc and when I reveal I see nothing, after reincubate, or incubating with a new antibody, the signal is...
29 August 2023 9,130 3 View
Hello everyone. We are using thrombin from bovine plasma (T4648-1KU, sigma). ¿How can we make a stock of 100 U/ml thrombin (0.1% BSA solution)? ¿Should we weigh the powder and reconstitute it...
05 July 2023 2,691 2 View
I use 1x ttbs, wash it 4x for 5 mins and 3x for five mins after transfer, and before blocking. I use everyblot from biorad as the blocking agent and for antibody incubation. Thanks!
04 July 2023 9,343 3 View
I accept suggestions for articles about linalyl acetate films or membranes.
21 December 2022 2,163 0 View
Hi! I am using DADA2 and phyloseq in R and I get slightly different results if I run the commands more than once with the same sample. I am working with 16S rRNA gene amplicons (Illumina, single...
29 November 2022 4,079 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...
09 August 2024 7,892 0 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...
08 August 2024 719 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View