Does anyone have experience of tracing the tract of micro-electrodes implanted in cerebellum?

25 November 2013 0 9K Report

I have a few sections of cerebella. I was assigned to do some staining to trace the tract of these microelectrodes. I tried Perl's staining. The electrode tract could be observed only when there's blood. I stained also GFAP but the activated astrocytes are located in a large area which is not specific enough to locate the tract.

Does anyone have some suggestions?

Badges
Science topic

More Hugh H Chan's questions See All

How to solve this error while I simulate gidl using tcad ?

There is an error message "interface silicon/oxide not found in grid xxx.tdr file"

26 June 2024 5,551 0 View

How to eliminate imaginary phonon frequency in phonon dispersion of Coved Graphene Nanoribbons?

Dear all, I want to calculate the phonon dispersion of 4CNR-1-0 (structure is in Fig. 2 (b) in this article Designing coved graphene nanoribbons with charge carrier mob... ) and then calculate...

17 June 2024 6,911 2 View

Synthesis poly(N-isopropylacrylamide) through ATRP by using CUBr/PMDETA?

Monomer: NIPAM; Initiator: BiBB; Catalyst: CuBr; Ligand: PMDETA I added CuBr and the mixture was blue, after that, I purged with nitrogen for 30 minutes and the mixture turned green. The mixture...

27 May 2024 9,144 1 View

How to determine decision limit (CCα) according to CIR 2021/808?

CIR 2021/808 written as "the CCα shall be calculated as follows: Method 1: by the calibration curve procedure according to ISO 11843-1:1997 ( 14) (here referred to as critical value of the net...

13 May 2024 9,481 0 View

Time to vacuum the chamber of the E-beam evaporation system?

We bought the E-beam evaporation system without a loading chamber. until now, we have used this system about 20~30 times for the deposition of Al2O3, Ti, Au, and Al having thicknesses from 50~100...

23 April 2024 5,156 4 View

Testing methods to determine a non-gene-silencing enzyme inhibitor?

I've recently been trying to investigate the inhibition of H2S-synthesising enzymes: CBS, CSE and 3-MST, via PCR. We've ordered EGCG, PAG and i-3MT-3 to inhibit each enzyme respectively, and have...

16 March 2024 392 1 View

What are the statistical approach for servant leadership in F&B?

To have multiple case study exploration among Malaysia restaurants that study the impact of servant leadership, what are the best statistical approaches for the case studies?

01 March 2024 2,737 1 View

How to reduce the collapse of microfluidic chamber?

During the fabrication of the microfluidic chamber (check its structure in the file, a 5x2.4mm microfluidic chamber with height of 20 μm), the ceilings of the chamber often collapse when bonding...

27 February 2024 6,330 3 View

Why it is necessary to dilute the concentration of surfactant during the process of crosslinking of droplet microfluidic produced calcium alginate?

I see in some references like doi: 10.1021/acs.analchem. 1c02335 and /10.1016/j.colsurfb.2022.113114, mention that it is necessary to dilute the concentration of surfactant during the process of...

27 February 2024 9,571 2 View

Continuing PCR with contaminated reagents?

I want to test out some new primers, but I know from past PCRs that at least one of my reagents is contaminated. I'm on a time crunch to finish my project and am not sure if I can get new reagents...

21 February 2024 4,799 0 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

Turning the Brain into a Vegetable: What does it tell Us?

A vegetative state is caused by having massive damage to the mesencephalon, which can include both the pontine nuclei and the thalamus (Plum and Posner 1980). During this state, the metabolic...

23 July 2024 6,177 0 View

Co-culture of SH-SY5Y cells and astrocytes?

Has anyone tried co-culturing and SH-SY5Y cells with human astrocytes and differentiating ? I am planning to seed astrocytes, let them grow for 3 days and introduce SH-SY5Y cells into it. Ideally,...

01 July 2024 243 2 View

How does the brain tell time (insights vis-à-vis Stephen Hawking)?

In 1995-96, when Andreas Tolias arrived at MIT to work in the laboratory of Peter Schiller, it was clear that he would become an exceptional neuroscientist. One of the first recommendations...

24 June 2024 7,831 0 View

Does the temporal summation of EPSPs accumulate linearly to action potential threshold or logarithmically to a plateau/limit?

I am attempting to determine which current inputs (in uA) are subthreshold for inducing a burst of activity. This is because I am attempting to model DBS form epilepsy in vitro, which clinically...

23 June 2024 4,083 1 View

Study of Efference-copy Mechanisms will Eclipse the Study of Attention?

Receiving reviews of a paper (Tehovnik, Carvey et al. 2003) in which we electrically stimulated the visual cortex of primates to evoke saccadic eye movements under various behavioral conditions to...

19 June 2024 4,676 0 View

What are the best criteria for detecting Healthcare associated infections, especially in case of urinary tract infections?

This is an important step for isolation of hospital pathogens.

18 June 2024 5,633 1 View

Mammalian neocortex supports a steady stream of consciousness?

We are taught in elementary statistics that a correlation does not prove causation. Nevertheless, given that the number of neurons in the neocortex scales at the slope of one with respect to the...

07 June 2024 1,815 0 View

The Cerebellum and Efference Copy Control?

“Theoretically, the computation of passive motion requires a comparison between an internal estimate of the sensory consequences of active self-motion (i.e., forward model) and the actual sensory...

03 June 2024 3,052 0 View