Protein assay to use with human cell phosphoproteins (phosphoAKT) prior to western blot.

I am trying to detect phosphoAKT in human neutrophils using a Western blot. I have lysed my cells using MOPS-based buffer (MbB), Dithiothreitol (DTT), Protease Inhibitor Cocktail (PIC),...

02 February 2016 6,311 6 View