Many cell lines as well as primary cells could grow without a functional oxidative phosphorylation (rho zero cells). This is done primarily by depleting mitochondrial DNA using ethidium bromide or using nucleases targeted to mitochondrial DNA. Did anyone develop rho zero adipocytes or preadipocytes successfully? I would appreciate sharing your experience with these cells!
Rudolf J Wiesner
making cells rho zero is an EXTREMELY unlikely event, since mutations in the nucleus have to occur as well so cells can survive wo a respiratory chain - thats why so few lines are around !! But you can try to make them pretty much rho minus with EtbR
1 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line
Similar questions and discussions