We've been trying to avoid using RIA kits or methodology using I125.
How can I analyse the relationship between five independent variables and one dependent variable, with a moderating variable influencing each of these five relationships? How can I test hypotheses...
03 June 2024 6,221 1 View
We are experiencing different problems pelleting fibroblast: a) From the resuspension of the comercial vials (after defrosting, we resuspended in 5ml complete DMEM + L-Glutamin + FBS +...
28 May 2024 3,529 1 View
We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....
28 May 2024 6,052 2 View
Hello. I am here looking for help with the NO radical scavenging assay. I was working on setting up the assay with sodium nitroprusside and the Griess reagent as they do in most works. However, I...
27 May 2024 5,013 1 View
Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...
21 May 2024 2,171 0 View
We have created a humanized mouse model using transplantation of human cd34+ bone marrow cells. I want to make mutations in genes prior to transplantation. What kind of selection markers are used?...
20 May 2024 577 0 View
Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.
16 May 2024 9,432 1 View
I want to evaluate the polarization to M2 phenotype in Raw Cells, I co-cultured the cell with LPS and my drug to see the effect of the drug to inhibit the proinflammatory effect of LPS, but I have...
05 May 2024 8,525 0 View
Sirs. ResearchGate Receive a cordial and respectful greeting. For academic purposes only, we request collaboratively the following articles for one of our research professors, who is preparing...
05 May 2024 9,459 9 View
How can we prevent small peptides (1000 Da) from eluting out of the gel during staining in a peptide gel (tricine-SDS-PAGE, Shagger)? There are different types of fixing solutions, but I'm not...
28 April 2024 1,522 0 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.
06 August 2024 6,796 3 View