Does anyone have experience blocking plant contaminant DNA during PCR?

03 March 2014 0 7K Report

I want to design a blocking primer to enhance the amplification of rare individuals in the phyllosphere. Some papers report the use of a blocking primer with a 3' modification called C3 spacer.

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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