Hi,
I know the most used host are insect cells, but Id like to see if this is feasible in yeast.
Thanks
HI,
It depends on which cyclin/CDK you are going to study. You need to find out if your cyclin/CDK is less homolog to any cyclin/CDK of the yeast you choose and if the molecular size of your cyclin/CDK is different with the one of the yeast. Then, you would not get problems when you make mutants, analyze the genes or seperate the proteins.
L. Hu
I tried with CDK1/Cyclin B with succes. It depends on the complex you're interested in, I guess, if they're stronger enough to form de complex, but maybe you can look for 2-hybrid results to get an idea if you can do it with your proteins of interest.
You can use yeast to express whatever you want the only thing that you have to keep in mind is that you must codon optimize the sequence.
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