23 September 2022 3 8K Report

I need a reference about normal values of selenium in muscles of lambs in order to compare with my study with Pelibuey lambs

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Why do open and free science in a world where science is not open and free?

Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.

01 August 2024 10,046 1 View

Why Is My mtDNA Ct Value Higher Than Nuclear DNA in qPCR?

Hello everyone, I'm currently working on calculating the relative levels of mtDNA using qPCR by comparing the Ct values of a mitochondrial gene (nd1) and a nuclear gene. According to my...

16 July 2024 5,508 0 View

Is it possible that the Earth's axis of rotation could change?

Due to the shifting ocean currents, the planet could be thrown off balance, altering the angle of rotation, and even causing the axis to shift, plunging us into chaos. In reality, it doesn't take...

15 July 2024 8,949 1 View

How to Freeze Embryos for Spatial Transcriptomics?

Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...

10 July 2024 5,985 0 View

Are there any information about protocols of CRISPR in leishmania strains?

I am looking for a CRISPR protocol for application in leishmania strains

08 July 2024 9,732 1 View

¿En qué medida las tecnologías educativas están siendo efectivas para promover el desarrollo de habilidades para la vida?

las

19 June 2024 2,125 3 View

Expert in advanced statistics interested in contributing to an academic article on the adoption of digital innovations in agriculture?

We are looking for a highly qualified researcher with expertise in advanced statistical analysis to contribute to a scientific article to be submitted to a prestigious journal by the end of the...

13 June 2024 569 4 View

Does wind power turbine use increase world temperatures a lot and create hurricanes?

Wind turbines extract a lot of energy, about 30-35%, that passes through. Now they are building a lot of them, more and more powerful. Change wind trajectory is easy, if a lot of obstacles is in...

12 June 2024 9,002 2 View

Is eFluor™ 670 eBioscience stain lost when cells die? Could Hoechst stain affect NK cell functionality?

Dear all, I am using Is eFluor™ 670 eBioscience die (Invitrogen 65-0840-85) to stain NK cells. The datasheet indicates that it binds to any cellular protein containing primary amines and that it...

29 May 2024 5,881 0 View

Any experience in fibroblast centrifugation?

We are experiencing different problems pelleting fibroblast: a) From the resuspension of the comercial vials (after defrosting, we resuspended in 5ml complete DMEM + L-Glutamin + FBS +...

28 May 2024 3,529 1 View

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Maximum isometric force of shoulder muscles?

Are there any references that report the maximum isometric forces of shoulder muscles?

08 August 2024 6,060 0 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

Does anyone have sources where I can find the contraction ratios (% shortening of muscle length during full contraction) of specific muscles?

I founde some textbooks stating that typical skeletal muscles can shorten up to 70% (optimally up to 30%) during contraction. However, I would like to look at muscles per region. This might seem...

21 July 2024 399 3 View

How is skeletal and respiratory muscle ultrasound being used in the clinical setting, and what are the barriers and facilitators?

Are you a qualified health professional working in the acute adult setting? If yes, then we want to hear from you! Link to survey: https://q.surveys.unimelb.edu.au/jfe/form/SV_aWORJRowoBvxC1U We...

15 June 2024 7,978 1 View

Litopenaeus vannamei post-larvae for fatty acid and amino acid profile?

For fatty acid and amino acid profile analysis in Litopenaeus vannamei post-larvae (1-1.5 gr), is it necessary to remove the head and peel? or it's possible to use whole body including head, peel...

04 June 2024 4,497 0 View

Could diaphragm spasms cause obstructive sleep apnea and pulmonary hypertension?

Hypothesis: Diaphragm spasms contribute to obstructive sleep apneas and pulmonary hypertension. While in diaphragm spasm, which appears to happen most often in REM-sleep (in the form of...

30 May 2024 297 4 View

When consider peroneal muscles active during drop jump landing?

Good morning everyone, I'm conducting some trials with sEMG located on peroneal muscles: subjects are making a drop jump and a re-jump immediately after landing on the same leg. I haven't got a...

21 May 2024 4,924 4 View

What are the main Sensations and Symptoms in fibrilar rupture of the Abdominal Muscle in Tennis Players?

I need to know what are the main sensations and symptoms, tennis players feel when their abdominal muscle suffers this type of injury. Thank You

20 May 2024 1,454 0 View

What would you do if you were a Farmer and asked to convert your farm from meat to plant productions?

Dear Researchers: I'm wondering if you were a Farmer raising cattle or poultry to earn monthly income for yourself and your family, and could have been asked to convert your farm to a wheat,...

03 April 2024 5,599 21 View

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