I have small query regarding RNA and RT-PCR. Within how many days do we need to do the expression studies after isolating RNA.?
Once Your RNA is stored in -80C, it can be kept for a while. However, I'd suggest to transcribe the RNA into cDNA first, and then it can be kept for a long time, as DNA is much more stable than RNA.
thank you Eitan
one year old samples also can be used? what is the time limit?
Hi, I agree with Ethan. For me, the sooner, the better. However, if you store your RNA at -80ºC it will work fine, provided the RNA is not so degraded. If you want to use it for RT-qPCR it should work even with some level of degradation. Before you start, you can electrophorese 1 uL of it to see the integrity pattern, or test the amplificability directly by using an andogenous constitutively expressed gene. Good luck!
I'm agree with previous suggestions, just to add more tips, you can storage RNA in precipitation solution (with ethanol 100% plus NaAc) years at -80 and also you can storage inmediately after you have added trizol or any Tri-reagent and place it at -80.As routine, I storage my samples as cDNA because I'm going forward as soon as I can, but maybe if your need collect many sample at different times you should consider previous comments. Anyway, if you already have done the RNA extraction, once you have measured RNA concentration, you can make aliquots and storage at -80C. I know that it more time consuming, but with precious samples sometimes is worth to try it Good Luck!
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