Kanakala S.

7 Questions 12 Answers 0 Followers

Questions related from Kanakala S.

How do you calculate or take equal concentration of RNA for cDNA synthesis?

There is a RNA concentration difference between gel and nano drop reading. This is reflecting my RT-PCR results. Nano drop concentration of all the four samples, 1. 1001.26, 2. 976.92, 3. 1061.99...

08 August 2014 5,775 10 View

Can anyone help me in finding a reference gene?

In real time PCR, housekeeping genes, 18S, beta tubulin, cyclophilin, actin, elongation factor1α/β, found not stable under stress (tomato flowers at 40 degree). How to find/standardize a reference...

08 August 2014 979 6 View

Does anyone have any experience with RT-PCR and RNA?

I have small query regarding RNA and RT-PCR. Within how many days do we need to do the expression studies after isolating RNA.?

08 August 2014 4,236 5 View

In RNA isolation does anyone know what will happen if isoproponol instead of chloroform is added to Trizol- tissue?

07 July 2014 5,110 2 View

Can anyone help me in standardizing an endogenous gene?

In realtime PCR, my aim is to standardize the stable gene in tomato tissues (control and heat stress). I am facing a problem finding a stable gene expression in both control and heat stress. I...

07 July 2014 357 5 View

Can anybody identify the plant ?

Name of the plant

05 May 2014 1,498 44 View

How can I avoid tissue damage?

My aim is to collect flowers from detached tomato branches after four days. For this, I am keeping tomato branches in water until four days. After three days, part of the branches which were...

04 April 2014 9,460 2 View