Does anyone have an optimized protocol for electroporation human dermal fibroblast with Lonza 4D machine?

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Should a "redundant" subclass relation be present in an OWL ontology after reasoning?

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How to identify and fix issues with a non-positive definite covariance matrix error in CFA using lavaan (without negative values in the cov matrix)?

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Could you kindly fill in Questionnaire: How to improve Refurbishing/Recycling of medical devices and tools in times of COVID-19?

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Culture fibroblasts with or without FGF2?

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Han Zhang

Hi Carsten, I once used electroporation in leukemic cells, and the transfection efficiency is always around 20%-30%. If adherent cells, lipo-transfection or virus-mediated transfection is recommended.

Carsten Charlesworth

In case anyone stumbles on to this thread program CA-137 achieves 90-100% transfection using GFP mRNA in human dermal fibroblasts. It also works pretty well for every other adherent cell type I've tested including: mESCs, hESCs, iPSCs and primed mESCs