Carsten Charlesworth

4 Questions 3 Answers 0 Followers

Questions related from Carsten Charlesworth

Does anyone have an optimized protocol for electroporation human dermal fibroblast with Lonza 4D machine?

Hi, So I am trying to electroporate human dermal fibroblasts using the Lonza 4D nucleofector however the electroporation program that lonza recommends gives less than 20% GFP positive cells...

22 January 2018 765 2 View

Anyone Had Success Concentrating AAV vectors?

Hi so we have been producing AAV vectors in our lab for some experiments however no one in our lab has been able to concentrate the final virus prep. We have tried both AMICON and VIVASPIN...

28 July 2016 492 7 View

Optimal Conditions For Using Serum As A Primary Probe For Western Blotting?

Hey, So I have been working on a project trying to detect whether we have antibodies against a certain protein present in our serum. I have been getting some good results however I'm not sure what...

03 June 2016 6,701 1 View

Optimal Conditions for Storing Human Serum at 4C?

Hi I am immunoblotting looking for an antibody in human serum. Storing at -20C and -80C damages the antibody so I need to store the serum at 4C for the duration of use. I was wondering what the...

19 April 2016 3,054 3 View