Does anyone have an idea what a highly expressed, 30.000 da protein in turtle (T.scripta) skeletal muscle could be?

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Signe Helbo

Hi Arli,

I don't have the sequence so that's not an option... I can just see it on an SDS gel.

Alexander Streng

I am guessing you are looking at Troponin T.

That protein is highly expressed in muscle tissue and is about 30-35 kDa.

But it is impossible to be sure without any identification experiments. Try blotting it with a skeletal troponin T antibody. Troponin T is very well preserved in its centre, so you may get lucky. Your best bet at identifying the protein is to cut it out and do a digest+mass spectrometry, if you have access to those techniques.