Hi,

I am currently trying to calculate the PCR efficiency for my qPCRs. I was using Taqman probes and was told that I could use the LinRegPCR software to calculate the efficiency, but as far as I could tell this would give me a bias, because the fluorescent is accumulating when using the TaqMan probes and this is not happening in the same way when using e.g SYBR.

The goal here is to compare the expression of my gene of interest to a housekeeping gene for a relative expression of the GOI.

Have any of you done something similar using Taqman and might have some input on how to do the calculation?

Thanks in advance.