Does anyone have an idea of what cell density HCASMCs ought to be seeded at to obtain immediate 100% confluence in a 6-well plate?

05 May 2016 0 8K Report

I'm trying to work out the density of Human coronary artery smooth muscle cells (HCASMCs), that when seeded in a well of 6-well plates, will be at 90-100% confluence. I need them to be fully confluent and therefore stop proliferating after plating before I can start my experiments.

Thank you in advance.

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