We are looking for a method to stabilize Heparansulfate (or carbohydrate side chains in general) during/after fixation of HUVECs with glutaraldehyde or PFA.
If anyone has an idea- please contact us!
Thank you very much! I will check this paper...
While computing the mechanism of a trimethylsilylcyanation related to this work (10.1021/acs.organomet.1c00070) I managed to optimize the geometry of both reactants and product and locate a good...
26 May 2024 9,925 0 View
Hi, I want to isolate nuclear proteins from the diatom P. tricornutum. I can successfully isolate the nuclei, and can image them under the microscope. But I fail to lyse the nuclei. As they were...
14 December 2023 3,640 1 View
I am looking for high molecular weight polystyrene for technical applications. The molecular weight should be at least 500.000 g/mol or higher. The PDI is not that important. Can anyone give me...
29 August 2023 7,516 2 View
Dear all, Our group has tried for several months to establish a homemade protocol for MDA-assay (Malondialdehyde Colorimetric Assay) to measure lipid peroxidation levels in cell culture. So far,...
29 August 2023 7,697 3 View
Dear all, I am curious to learn about the identification of the pictured salp individuum. The pictures were taken in October 2022 near San Antonio del Sur (southern Cuba) not far away from shore....
15 August 2023 9,008 0 View
hi community, I would like simulate the pump down graph of my vacuum pump. My first test is to use laminar flow, create an air cube of 1m^3 and add a vacuum pump on a side. The specificity of the...
13 June 2023 8,974 1 View
Hi, I would like to use mEOS to tag proteins to evaluate their subcellular localization. I am new to PALM, and am a bit confused with all the different versions of mEOS available... mEOS2? mEOS4?...
19 April 2023 7,456 1 View
Dear colleagues, On an expedition in the southern Sargasso Sea, subtropical Northwest Atlantic, I encountered repeatedly specimens of the photographed heteropod, never (so far) being bigger than...
28 March 2023 3,057 4 View
Hello everyone, For the quantification of a product (mix of 2 plasmids) by qPCR/ddPCR, we obtained inconsistent results between the different 10 fold dilutions in nuclease-free water (dilution...
07 March 2023 6,798 2 View
Hi, I was wondering if it is known if STORM microscopy could be used to obtain super-resolution images of sfGFP or mVenus tagged proteins in cells. I know it is more common to use fluorescent...
16 February 2023 982 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View