Florian Brettner

5 Questions 5 Answers 0 Followers

Questions related from Florian Brettner

Regarding fixation of HUVECs, is glutaraldehyde, glutardialdehyde, PFA, formaldehyde or ethanol better? And which concentration?

Has someone of you experiences with different fixation methods of HUVECs as described above? We have an immunofluorescence protocol for heparan sulfate staining (including fixation with 0,5%...

19 December 2014 3,608 3 View

Does anyone have an idea how to stabilize heparansulfate during or after fixation of HUVECs?

We are looking for a method to stabilize Heparansulfate (or carbohydrate side chains in general) during/after fixation of HUVECs with glutaraldehyde or PFA. If anyone has an idea- please contact us!

12 December 2014 5,316 1 View

Is there a protocol available for immunofluorescence staining of syndecan-1 in paraffin-embedded mouse hearts?

We use the Langendorff mouse model. Does anyone have a protocol for immunofluorescence staining of syndecan-1 in mouse hearts?

27 October 2014 3,908 1 View

How can I combine a primary antibody with an immunofluorescence secondary antibody?

I've one kind of primary antibody and one kind of secondary antibody and want to link them before I use them for the experiment. Has anyone an idea how to proceed?

04 April 2014 4,965 2 View

How to avoid autofluorescence in paraffin sections (of mouse hearts)?

I wanted to ask if someone has a suggestion concerning the avoidance of autofluorescence in paraffin sections of mouse hearts?

24 March 2014 712 1 View