Does anyone have an idea for the protocol and ideal key points to follow in isolation of cationic antimicrobial peptides?

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Q1: How can I improve hybridization and the process of hybridization?

Procedure: Streptavidin-magnetic bead suspension (50 µL) was transferred into a vial. The beads were washed twice by the binding/washing buffer and then re-suspended in 100µL of the buffer. A...

16 May 2024 893 0 View

I want this paper:Nonlinear hyperbolic equations and related topics in fluid dynamics?

who can send it to me?it is important for me.thanks.

14 May 2024 5,983 4 View

How to get research papers with their cites, and what are the keywords you have to put to get context/articles/papers you need?

06 May 2024 8,904 1 View

How to create unique visualizations for a manuscript? Reference attached?

Hi A paper titled " Intelligent Computing: The Latest Advances, Challenges, and Future" was recommended by my colleague. It has unique visualizations. I am attaching one of them for reference....

29 January 2024 3,880 1 View

How to retain viral injected fluorescence in paraffin embedded brain tissue in mice and rat?

We have injected GFP and td-tomato viral tracing injection in the mice brains to see axonal projections, and we are using tertiary butyl alcohol instead of ethanol for the dehydration process as...

09 October 2023 8,654 8 View

How to covert HCN gas emission from mg/kg to mg/Nm3??

Unit conversion for HCN from mg/Kg to mg/Nm3

27 September 2023 1,414 1 View

How to increase gain property in powerlab ADinstrument?

Hi everyone, I am doing auditory brainstem response measurements using powerlab AD instruments, but I am not getting the responses for lowest dB level as they are too weak to be measured by...

13 August 2023 3,223 0 View

For confocal microscopy, is there a way to tag ubiquitin protein without hampering its job in vivo?

Hello everyone! I am planning an experiment where I would like to check ubiquitin transfer using confocal microscopy; thus, I intend to add a fluorophore tag to the ubiquitin molecule. I read a...

12 August 2023 7,532 1 View

Have you identified any safety-related behaviour changes among the workers from unorganised sectors after the pendamic?

In my observation, during the pandemic in 2020-21 workers from unorganised sectors learned things to survive and adopted creative ways to complete their tasks. However, people also started...

01 August 2023 2,272 0 View

Can we convert kallikrein inactivation units (KIU) into unit/mL?

Aprotinin is used as a plasmin inhibitor. 126KUI helps to inhibit plasmin. We have 126 ph. Euro. U/mL concentration of aprotinin with 0.5mL vial. How much will KIU be there in a 0.5mL vial?

14 May 2023 3,348 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Arvind Negi

Follow these links:

http://www.mdpi.com/1420-3049/17/3/2980/pdf

http://www.jleukbio.org/content/56/5/661.long

Ondřej Nešuta

Hi,

try e.g., RP-HPLC on C18 column and gradient from 5 to 70% of acetionitrile with 0.1% TFA in 60 min. Detection at 220 and 280 nm.

Article Antimicrobial Peptide from the Wild Bee Hylaeus signatus Ven...