Why the storage moduli E' decrease with highr fiber volume?

Hi guys, I am facing problems to interpret a DMA results. Material: Composite Bisphenol A + curaua fiber What is being tested: the influence of fiber volume on the composites properties...

12 February 2021 174 7 View

Which buffer should I use to aliquot antibody in?

for western blot analysis the buffer in which the antibody already is, written in the datasheet, or should I use already the bsa or milk solution that I use for final diluition?

17 November 2020 4,804 3 View

What is the best protocol for simultaneous extraction and purification of DNA and RNA from animal tissues?

I need to perform a co-extraction of DNA and RNA from pools of juvenile Daphnia magna. I used the AllPrep DNA/RNA Mini Kit (QIAGEN) but with poor results in terms of DNA yeld and quality. I would...

22 October 2020 9,746 3 View

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The threat of pandemic disease is not new: For decades, public health experts advised that a coronavirus outbreak was looming, global proportions, and that the world was not equipped to fight it...

27 May 2020 2,871 25 View

Is it correct to compare Fst values obtained from different genetic markers?

Fst is used in many genetic studies which involve different markers (mtDNA, microsatellites, SNPs...) as methodologies and next-generation genetics improve. I was wondering if it would be correct...

27 April 2020 7,175 3 View

My work entails comparing the effect of tax on economic growth of two countries. Asides the panel regression, which other technique can I use?

MY specific objective is to make comparative analysis on the effect of tax revenue on GDP of country A and country B

26 June 2019 6,619 3 View

In Morris water maze training results for scopolamine-injected rats, is it normal that scopolamine doesn't cause difference on day 5 ?

And if so, is it related to short term memory or inability of scopolamine to impair spatial learning?

17 June 2019 9,461 3 View

How to prepare aqueous solution of Methyl Jasmonate dissolved in Tween 20?

I need to prepare 500 mL of aqueous solution of 1mM Methyl Jasmonate dissolved in 0.1% of Tween 20 for foliar application. How much MJ and how much Tween 20 do I need to use? Both compounds are...

02 May 2019 6,228 2 View

May I express a protein in C. glutamicum using a commercialy availabe vector?

29 March 2019 7,700 1 View

How can i use ethidium bromide for sample staining?

I don't want to add it directly into the gel but just using it as loading dye.

25 March 2019 5,889 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View