I culture isolated mouse islets at 27oC for the first 48h changing the media after 24 and 48h, then change the temp to 37oC (Zmuda 2011 JOVE). I have cultured islets happily for up to 3-4 weeks in non-TC treated dishes so they dont stick to the culture dish, but I find that they often stick to one another forming large, lobed islets which can develop necrotic cores. Does anyone have experience with this and perhaps found a way to reduce this issue?
Hi,
I am doing cell spheroids.
To prevent the cells to attach the culture dish, you pre-incubate the dishes with a solution of PVP (you could also use Poly-HEMA) prior the culture.
To prevent the cells to stick to each other, you could also add 0.4% BSA in your medium and will reduce the attachment intra-islets.
Hope that helped you.
Stephane
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