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I culture isolated mouse islets at 27oC for the first 48h changing the media after 24 and 48h, then change the temp to 37oC (Zmuda 2011 JOVE). I have cultured islets happily for up to 3-4 weeks...
06 June 2016 4,529 1 View
We have come across a strange issue with a western blot optimisation. We are probing for Akt (p). We keep (done multiple times, because I thought it was operator error) getting completely blank...
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