Hello all,
I am trying to asses the viability of stem cells in GelMA (gelatin methacryloyl). Most articles make their own GelMA. However, our team bought a commercially available one. The thing is : the product is fluorescent-green under the microscope, so it is difficult to evaluate the cell viability with the available staining kits, which show live cells also in green.
Thank you,
Dear João Luiz Monteiro,
I believed your current finding on GelMa is auto-fluorescence with green color thru specific wavelength. To my knowledge you could diminish or differentiate the auto-fluorescence thru the software and identify true wavelength for your vianlbility kit. It is totally challenging but could do that. Otherwise, you could using live cell tracker with different color as I believed your fluorescence color for dead cells is red in color? You could justify your procedure for some limitation, that's research.. Best of luck!
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