Dear Susanna,

 Please read the following:

http://www.uscnk.com/uscn/ELISA-Kit-for-Human-Myelin-Basic-Protein-MBP-214.htm

1-ELISA Kit for Myelin Basic Protein (MBP)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species Homo sapiens (Human)

Product No. SEA539Hu

Sample type Serum, plasma, cerebrospinal fluid and other biological fluids

Format 96T

Assay length 4.5 hours

Detection range 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL

Sensitivity The minimum detectable dose of this kit is typically less than 5.7pg/mL.

Specificity

This assay has high sensitivity and excellent specificity for detection of Myelin Basic Protein (MBP).

No significant cross-reactivity or interference between Myelin Basic Protein (MBP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Myelin Basic Protein (MBP) and the recovery rates were calculated by comparing the measured value to the expected amount of Myelin Basic Protein (MBP) in samples.

2-Human Myelin Basic Protein

(MBP) ELISA

For the quantitative determination of human MBP in

serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid,

cell culture supernates and other biological fluids

Cat. No. KT-22734

http://www.kamiyabiomedical.com/pdf/KT-22734.pdf

3-MBP myelin basic protein [ Homo sapiens (human) ]

http://www.ncbi.nlm.nih.gov/gene/4155

Gene ID: 4155, updated on 15-Nov-2015

Summary

Official Symbol

MBPprovided by HGNC

Official Full Name

myelin basic proteinprovided by HGNC

Primary source

HGNC:HGNC:6925

See related

Ensembl:ENSG00000197971; HPRD:01158; MIM:159430; Vega:OTTHUMG00000132874

Gene type

protein coding

RefSeq status

REVIEWED

Organism

Homo sapiens

Lineage

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo

Summary

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

Orthologs

mouse all

hoping this will be helpful,

Rafik

Rafik, thank you for your response. Have you used these kits before?

Dear Susanna,

Not yet.

I wish a good luck in your kits' work.

Rafik

Thank you, Rafik.

Did you get an answer/solution of your question?

As myelin basic protein binds tightly to the big alpha 2 macroglobulin it might be difficult to measure all MBP in Plasma. Maybe it is a better a idea to look for MBP-A2M complexes. I'm thinking to try to built up such an assay. What do you think, Susanna?