How to achieve a high efficiency DNA Strand Displacement?

29 September 2023 0 5K Report

Thanks for any help, this is my first time conducting DNA experiment. I want to hybridize a 26-base-DNA to 38-base-DNA and then use a 100-base-DNA to replace the 26-base-DNA, is this promising to achieve in room temperatue without enzyme?

Badges
Science topic

More Meng Xu's questions See All

Can anyone recommend a brand of cas9 protein that can be used for in-vivo editing in model animals?

zebrafish and mouse

19 July 2024 9,158 1 View

Dear scientific researchers, may I ask you, what is the function of the ∑ in the picture?

10 July 2024 2,037 3 View

Do the parts of the body that are situated at a longer distance from the central nervous system have the least control & coordination comparatively?

What would be the effect of distance from CNC cum Heart on the structure and function of a particular part of the human body? Do short people have evolutionary advantages to control body parts in...

09 July 2024 2,052 3 View

What are the trending topic to writ e a review article on it?

The topic should relate to pharmacy ,drug, methos of drug discovery, or any other topic but related to dug or pharmaceutical field

02 July 2024 4,031 3 View

Why are there holes on the surface of TiO2 films deposited by ion beam sputtering?

We have observed many holes on the surface of TiO2. These holes have a diameter of approximately 1 µm and a depth of about 30 nm. The formation of these holes is mainly related to the...

20 June 2024 3,861 1 View

How do you understand that geometric phases are complex?

What kind of cases are geometric phases real? In what case is the geometric phase complex? If the geometric phase is complex, how do we make sense of it?

18 June 2024 6,388 4 View

Why the rat cortical primary neurons suddenly dead/turn into bad condition after about 6days?

I used SD rats (E19) for cortical neuron extraction. Due to the need for subsequent special experiments, I cultured the neurons in a Corning PDL 384-well plate. The culture medium is Neurobasal +...

12 June 2024 1,714 4 View

Has anyone tested using Orbitrap as detector for ms2 in TMT SPS MS3 method?

Hello, In the standard TMT SPS MS3 method, ion trap is used as detector for ms2 data acquisition. In my experiment, it would be much beneficial to use Orbitrap for ms2 data acquisition to obtain...

26 May 2024 628 0 View

What does oxide content mean in XRF testing?

XRF tests were performed on magnetite where the oxide content showed 49.822% Fe2O3, so why not Fe3O4 content as a percentage? How can we deduce the Fe3O4 content?

25 May 2024 1,714 2 View

Can acoustic wave transfer through solid medium?

Since the acoustic impedances of air and solid medium are not in the same order, the acoustic wave transferring in the air would be theoretically fully reflected on the solid surface. So my...

21 May 2024 6,858 9 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there an alternative to a multinomial regression which allows the DV to be non mutually exclusive?

I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...

06 August 2024 1,752 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

How combine yolo with Faster R-CNN?

I want a model that is balanced with accuracy or speed, faster rcnn has high accuracy while yolo have fast speed. i am thinking to combine them to get a hybrid model to achieve both speed and accuracy

02 August 2024 3,104 0 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Talking therapies for bipolar, psychology?

what is the best research evidence for psychological interventions for Bipolar?

01 August 2024 6,023 2 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

Recruitment for Postpartum Mental Health Research?

We are currently recruiting for two studies on postpartum mental health as part of our work at the Perinatal Mental Health Research Lab at Alliant International University. If you or someone you...

30 July 2024 2,950 0 View

Entropy measure and QSPR modeling in Graph Theor. How to construct the table for lengthy equation?

The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...

30 July 2024 3,126 0 View