I have isolated some phages from sewage samples and need to determine whether they have RNA or DNA genomes. I understand that I should treat samples with RNAse/DNAse and check for lysis in an agar overlay, or extract the nucleic acid then treat with RNAse/DNAse and run a gel but I am struggling to find a protocol for either of these methods. I also am unsure whether a protease is needed prior to nuclease treatment?
Andrew D Millard
You could also try staining your samples with different dyes as this would also allow differentiation DNA or RNA . see UNIT 7.3 Differential Staining of DNA and RNA
Zbigniew Darzynkiewicz1, Gloria Juan1, Edward F. Srour2 . If you have already extracted the nucleic acid then there would be no need for a protease ,
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