Fibrin matrices seeded with cells
Hi, I am getting elevated results in my starch analysis. It is about 2-3 % higher than usual. We have not changed anything. We are getting the same results over and over. I have checked the...
25 March 2024 1,395 6 View
As an alternative to looking at the conservation of cysteine residues amongst homologs of a protein of interest, is anyone aware of a server that can predict whether disulphide bond formation...
21 March 2024 3,803 1 View
I am trying to purify a his tagged protein using IMAC. The homologs of this protein purify very well. However, this protein precipitates on elution. Buffer composition- 20mM Tris-HCl pH7.4, 500mM...
20 March 2024 9,095 4 View
I have purified several his tagged proteins using IMAC. To clean up the samples further I ran them through a GF column (Superdex 200 increase 10/30Gl). When I ran an SDS sample containing the...
19 March 2024 4,202 3 View
Hi, Has anyone got a method to test glycoalkaloids in potato. I don't have an HPLC so I'm looking for a wet chemistry method. I would like to quantify the results. If possible, I want to look a...
13 November 2023 1,668 1 View
We use an I house sample as a control sample (set up by running a PT sample along side it). However, over a few months it becomes rancid and we have to start the process again. Does anyone know of...
27 July 2023 8,679 0 View
Currently exploring similarities around the Design Thinking, Inquiry Learning, and Mathematical Modelling framework.
17 May 2023 8,746 1 View
as stated
18 February 2023 7,264 1 View
Folks, Does anyone know of a paper showing binding affinity data of DNA oligos for their complement? Ideally it would show data from a 'non-molecular biology method' such as ITC, SPR, or MST. Even...
12 January 2023 2,965 2 View
Can you use the chi-squared test if subjects from the groups in the predictor variable can appear multiple times in the response variable categories? & Can you use chi-square if you do not...
21 July 2022 7,061 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
