What statistical analysis method should I use with multivariate categorical data?

Hi, I'm struggling quite a bit, Hope someone can help. My research question is roughly to what extent (and what) characteristics of murder and manslaughter cases are related. The data consists...

05 October 2020 3,958 8 View

Problems with Bovine Fibrin Clots?

Hi I am using Fibrinogen (10mg/ml) and Thrombin (1000U/ml) to form fibrin clots. I found that after getting good cross linking initially the clots dissolve to liquid if left in incubator...

26 May 2016 9,416 4 View

When I added the interaction terms, the previously identified significant variables became insignificant?

I'm running a logistic regression test. On my first test, I've identified 6/7 significant explanatory variables. I then ran another logistic regression test with all the interaction terms. The...

05 April 2016 7,655 3 View

Anyone have a good methodology for Agar Gel for adding on top of a cell culture dish (and that allows you to pour/pipette as a thin layer)?

07 February 2016 7,189 1 View

For Fibrin Matrices is >2000 NIH/ml best - or can one use 600-2000NIH/ml in order to produce 1000U/ml concentration (Thrombin from Bovine Plasma)?

- used for making matrices to seed neonatal fibroblasts.

02 February 2016 8,338 1 View

What is the ultimate purpose of Ecology, and what do we hope to achieve in advancing the field?

01 February 2016 9,757 25 View

In what way can spirituality be experientially incorporated into Early Years Education in the UK?

21 May 2015 7,674 4 View

How can I find out the interaction of an osteoarthritis patient's DNA with Vitamin B complex?

I have osteoarthritis patient DNA. Now I want to study the interaction of the DNA with Vitamin B complex. What is the procedure to do so? What types of materials and methods are used to study this...

26 November 2014 1,981 2 View

How can I identify the subtypes of HLAB27 when I used Real-TM PCR which show qualitative results?

I used Real-TM PCR which shows qualitative analysis for identification of HLA B27 after DNA extraction from patient blood sample. The analysis show me whether it is positive or negative. But I...

12 October 2014 3,675 1 View

What is the process of calculating Rf factor from SDS-PAGE after protein extraction?

protein extraction

08 October 2014 7,028 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View