I am interested in isolating endothelial cells from mice and zebrafish spinal cords. I would appreciate some comments and tips regarding methodology.
Thanks!!
I have a protocol for isolation of endothelial cells from spinal cord. Write me a message and I will send it to you!
Asked 1 minute ago 2x=0 1/2*2x=0 X=0 Multiplica se o inverso do elemento que multiplica x em sua nulidade (x=0)
01 July 2024 2,814 2 View
The role of gut microbiota and diet has become a major concern for the health of Alzheimer’s disease (AD) individuals. The literatures shows that the microbiome composition has been linked to...
23 June 2024 9,503 1 View
The literature shows that gut microbiota plays a significant role in the development and progression of liver diseases. Research suggests that a disturbance to the gut microbiome leads to hepatic...
21 May 2024 5,967 2 View
1. Researchers highlight that they are the first to report on the function and applications of the CRISPR–Cas9 system. The authors explain how the CRISPR technology in clinical practice could be...
16 May 2024 7,017 0 View
More specifically: We are working on breeding some mycophagous Erotylidae and I would be very interested in obtaining eggs for life cycle studies. Thanks
20 April 2024 9,785 1 View
Hi all, In the framework of a LCA of perovskite solar cells, I am looking for information about scotch tape, that is used to recover the metals deposited in some layers. Does anyone know of...
18 April 2024 1,678 0 View
É por causa que é pouca à quantidade de sangue amostrada ou alguma enzima digestiva?
11 April 2024 1,112 0 View
Shadows could be a little energy and do not “without anything”?
30 March 2024 7,426 1 View
All or nothing is relative in terms of the form of knowledge. Nothing for me is everything you used to produce capital and have a dignified life. Everything is incomplete because every day new...
29 March 2024 8,857 0 View
Biotherapeutics and nutritional biotherapy have become important to reverse global chronic diseases. The role of Indian spices and biotherapeutics in health and chronic disease has become of major...
05 March 2024 9,530 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View