Calculation the Pump power needed in a pressurized flow system?

Hi all, I am design now a liquid high pressure flow system to be used up to 50 bar, and I am having some doubts concerning how I can estimate the pump power. Besides the pressure drop in the...

15 December 2020 8,411 1 View

Design of a membrane system to remove CO2 from a flue gas current?

Hi all, I am trying to design a membrane system to remove the CO2 from a flue gas current, and I am having difficulties in finding information. Does anyone have any technical information,...

15 December 2020 3,655 2 View

Can the potential of LIPIDMICS improve the diagnosis and understanding of metabolic changes in various neurodegenerative disease (PD, HD, AD, MS, etc?

08 November 2020 7,209 1 View

What pressures should I choose in a reheated rankine cycle?

09 October 2020 9,117 4 View

I want to measure cytokine levels in mouse spinal cord tissue and I can not decide which method is best, a cytokine array or ELISA?

Hi everyone. So I need to measure different cytokines in mouse spinal cord tissue and I can not decide which method is the best one, a cytokine array or ELISA. Does anyone that has experience in...

26 July 2020 1,757 2 View

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Nutritional science has provided functional foods with the required amounts of natural antioxidants, vitamins, fats, proteins, polyphenols, carbohydrates, and other components required to maintain...

03 June 2020 7,377 1 View

Is there any protocol capable of simulating an epileptic seizure in vitro using direct electric stimulation?

I am trying to simulate epileptic seizure activity in cell cultures by using direct electric stimulation. However, I am struglling to find an acessible and relatively easy-to-use protocol. Thank...

28 January 2020 4,951 2 View

How can I map brain in live rats after a certain stimulus without an imaging technique?

I need to look for brain rat regions activation after a certain stimulus made to live animals. The marker to be looking for needs to be maintained fro long periods because the stimulus that I need...

10 December 2019 8,626 2 View

How to avoid non-negative solutions in ODE solvers in R?

I'm using the package deSolve in R to model a biological ODE system, however I have the problem of, with certain parameters or with certain initial conditions, the ode solver produces negative...

09 December 2018 7,114 6 View

Are advances in Anti-Aging Research Therapy to reverse cell death critical to Global Anti-Aging Markets expected to EXCEED $331 billion by 2021?

RELEVANT REFERENCES: 1. Martins IJ. Advances in Aging and Health Research. Scientific Research Publishing, Inc. ISBN: 978-1-61896-569-1 2. Martins IJ. Scientific Nutritional Health and Global...

11 September 2018 2,602 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View